FastQCFastQC Report
Wed 5 Jul 2017
FCHGVKNBBXX-HKZEBggcRAAGRAAPEI-209_L3_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHGVKNBBXX-HKZEBggcRAAGRAAPEI-209_L3_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35907057
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG21850.021.9474120
TCGATCG14650.020.120783
CGATCGA14700.019.6035734
CTACCGA25700.016.34856823
TCTAAGG31050.016.0820918
CCGCGTA16500.015.73189927
CACTCGA43400.015.2566094
ACTCGAC39050.015.2098185
GCGAGTG33550.014.62149624
CGCCTAC55800.014.58632117
TCGCGTA7100.014.56205328
CCGCCTA56100.014.54753216
CGTACTA21150.014.45821735
CCACTCG44850.014.2237213
CCGAGCG105950.014.11934838
GGTAGGT65500.014.03831810
CTCAGAT194800.013.7502561
CGAGCGC109050.013.7380339
CTCGCAT53200.013.6447421
GCGCACC109500.013.62130242
Produced by FastQC (version 0.11.5)